Lysov Iu P, Mironov A A, Gnuchev F N, Chernyĭ A A, Mirzabekov A D
Mol Biol (Mosk). 1994 May-Jun;28(3):652-7.
DNA sequencing by hybridization on oligonucleotide matrix (SHOM) makes use of a matrix of immobilized oligonucleotides. Yet the method is not directly applicable for sequencing of fragments with long monotonous repeats. Measurement of the distances between certain segments within the DNA fragment analyzed provide additional information for sequencing. This information can be obtained by digesting DNA with a set of restriction endonucleases, or by PCR with primers complementary to certain DNA regions, with subsequent measurement of the length of the resulting fragments in gel electrophoresis. Use of this additional information increases the reconstruction efficiency and in many cases solves the problem of repeating and monotonous segments within analyzed DNA fragment. The current work presents the use of this information and the estimated efficiency of its usage.
基于寡核苷酸矩阵的杂交DNA测序法（SHOM）利用固定化寡核苷酸矩阵。然而，该方法不适用于具有长单调重复序列的片段测序。分析DNA片段内某些片段之间的距离测量为测序提供了额外信息。该信息可通过用一组限制性内切酶消化DNA，或通过使用与某些DNA区域互补的引物进行PCR，随后在凝胶电泳中测量所得片段的长度来获得。使用这些额外信息可提高重建效率，并且在许多情况下解决了分析的DNA片段内重复和单调片段的问题。当前工作展示了这些信息的使用及其使用效率的估计。
