Tret'iakov A N, Gel'fand V M, Pantina R A, Shevtsov S P, Bulat S A
Mol Biol (Mosk). 1994 May-Jun;28(3):665-9.
Amplification of 200-1000-bp DNA fragments was performed in 15-30 min using a rapid thermal cycler based on the commercial instrument TC-1000-1 (IRLEN, St. Petersburg, Russia). Plastic pipette tips were used as thin walled, high surface to volume-ratio tubes, to increase the rate of heating (cooling) of 20 microliters samples, which allowed the time required for DNA amplification to be considerably reduced (5-10 times).
