Mielicki W P, Tagawa M, Gordon S G
University of Colorado Health Sciences Center, Denver 80262.
Thromb Haemost. 1994 Apr;71(4):456-60.
A new, sensitive and specific immunocapture enzyme (ICE) assay for quantitation of the enzymatic activity of cancer procoagulant (CP) has been developed. The assay had good reproducibility (inter- and intra-assay CV were 6.4% and 5.7% respectively) and was linear for concentrations of CP from 0.5 microgram/ml to 10 micrograms/ml (r2 = 0.995). Using this assay the inhibition of CP by iodoacetamide, mercuric chloride, E-64, leupeptin and antipain was demonstrated. There was no significant effect of cystatin and natural plasma proteinase inhibitors alpha 1-antitrypsin, alpha 1-antichymotrypsin, alpha 2-macroglobulin and antithrombin-III/heparin, on the activity of the CP.
已开发出一种用于定量癌促凝剂（CP）酶活性的新型、灵敏且特异的免疫捕获酶（ICE）测定法。该测定法具有良好的重现性（批间和批内变异系数分别为6.4%和5.7%），并且在CP浓度为0.5微克/毫升至10微克/毫升时呈线性（r2 = 0.995）。使用该测定法证明了碘乙酰胺、氯化汞、E-64、亮抑酶肽和抗蛋白酶对CP的抑制作用。半胱氨酸蛋白酶抑制剂和天然血浆蛋白酶抑制剂α1-抗胰蛋白酶、α1-抗糜蛋白酶、α2-巨球蛋白及抗凝血酶III/肝素对CP的活性无显著影响。
