Chesne P, Heyman Y, Peynot N, Renard J P
Unité de Biologie du Développement, Jouy-en-Josas, France.
C R Acad Sci III. 1993;316(5):487-91.
The birth of a clone of five bull calves, each produced by fusing a cell taken from the same young embryo with an oocyte whose own nucleus has been removed, opens the way to the use of these new animal models for research and animal selection. The embryonic cells came from a 31-cell morula. Our results indicate that most of the nuclei at this stage are totipotent which seems to no longer be the case for those taken from the cells of the inner cell mass at the blastocyst stage.
通过将取自同一早期胚胎的细胞与去除自身细胞核的卵母细胞融合，培育出了五头克隆公牛犊。这为利用这些新的动物模型进行研究和动物选育开辟了道路。胚胎细胞来自一个31细胞的桑椹胚。我们的结果表明，这个阶段的大多数细胞核具有全能性，而取自囊胚期内细胞团细胞的细胞核似乎不再如此。
