Shen R N, Wu B, Lu L, Kaiser H E, Broxmeyer H E
Department of Medicine (Hematology/Oncology), Indiana University, School of Medicine, Indianapolis 46202-5121.
In Vivo. 1994 Jan-Feb;8(1):59-63.
Recombinant human Interleukin-1 Alpha (rhu IL-1 alpha) was assessed for its efficacy in modifying the immunosuppression of mice compromised by Cyclophosphamide (CY), retrovirus infection or surgical stress. Sublethal dose (300 mg/kg) of CY caused neutropenia, decreased cellularity of bone marrow and inhibited Natural Killer (NK) cell activity and lymphokine-activated killer (LAK) cell activity in DBA/2 mice. A single dose of rhu IL-1 alpha (1000 units/per mouse) i.p. accelerated recovery of blood neutrophils and bone marrow cellularity and restored NK and LAK cell activity in CY-treated mice. Mice infected with Friend Virus Complex (FVC) had decreased percentages of L3T4+ cells and a reversed L3T4+/Lyt-2+ ratio; NK and LAK cell activity also decreased. These impaired cellular parameters were restored by rhu IL-1 alpha treatment (1000 units/per mouse/daily i.p. starting on day 5 for 5 days). NK and LAK cell activity was impaired by surgical stress. A single dose of rhu IL-1 alpha (1000 units/per mouse) i.p. 20 hours before transfemoral amputation restored NK and LAK cell activity to normal levels in these mice. These studies indicate that rhu IL-1 alpha possesses immunomodulatory effects in vivo for a broad range of stresses.
对重组人白细胞介素-1α（rhu IL-1α）在改善因环磷酰胺（CY）、逆转录病毒感染或手术应激而免疫抑制的小鼠方面的功效进行了评估。亚致死剂量（300mg/kg）的CY导致DBA/2小鼠出现中性粒细胞减少、骨髓细胞数量减少，并抑制自然杀伤（NK）细胞活性和淋巴因子激活的杀伤（LAK）细胞活性。腹腔注射单剂量的rhu IL-1α（每只小鼠1000单位）可加速CY处理小鼠血液中性粒细胞和骨髓细胞数量的恢复，并恢复NK和LAK细胞活性。感染了弗氏病毒复合物（FVC）的小鼠L3T4+细胞百分比降低，L3T4+/Lyt-2+比值逆转；NK和LAK细胞活性也降低。通过rhu IL-1α治疗（每只小鼠1000单位/天，从第5天开始腹腔注射，持续5天）可恢复这些受损的细胞参数。手术应激会损害NK和LAK细胞活性。在经股截肢前20小时腹腔注射单剂量的rhu IL-1α（每只小鼠1000单位）可使这些小鼠的NK和LAK细胞活性恢复到正常水平。这些研究表明，rhu IL-1α在体内对广泛的应激具有免疫调节作用。
