McKenna M C, Muchardt C, Gaynor R, Eisenberg D
Molecular Biology Institute, University of California at Los Angeles 90024.
Protein Expr Purif. 1994 Apr;5(2):105-11. doi: 10.1006/prep.1994.1016.
A procedure leading to a 100-liter fermentor culture of Escherichia coli cells expressing the human immunodeficiency virus type 1 (HIV-1) trans-activator (Tat) protein is described. The effects of growth temperature and of cell density at the time of induction on the yield of Tat were investigated. Tat was identified by SDS-gel electrophoresis and Western blot. Tat represents approximately 10% of the soluble protein in the cell lysate.
描述了一种用于培养100升表达人类免疫缺陷病毒1型（HIV-1）反式激活因子（Tat）蛋白的大肠杆菌细胞的方法。研究了生长温度和诱导时细胞密度对Tat产量的影响。通过SDS凝胶电泳和蛋白质免疫印迹法鉴定Tat。Tat约占细胞裂解液中可溶性蛋白的10%。
