Stewart J, Rajabi H
Department of Psychology, Concordia University, Montréal, Que., Canada.
Brain Res. 1994 May 16;646(1):157-60. doi: 10.1016/0006-8993(94)90070-1.
We reported previously that exposure to testosterone (T), neonatally, slows the time-course of development of catecholaminergic activity in the anterior cortex of rat pups. In the present study we assessed the role of T in prenatal life on this development and explored the role of the estrogen metabolite of T, estradiol, in these actions. Male pups born to dams injected daily with 5 mg/kg, s.c., of the aromatase inhibitor 1,4,6-androstatriene-3,17-dione (ATD), or vehicle only, between gestation days 10 and 21 were either gonadectomized (GX) or anesthetized, only, within 6 h of birth. Dopamine, DOPAC, and noradrenaline levels were assessed, using HPLC-EC, in punched samples taken from cingulate (CING), agranular insular (AID), parietal (PAR) and occipital (OC) cortex on postnatal (PN) days 4 and 10. At PN4 there were no effects of treatment on amine levels, although there were higher levels in frontal areas. At PN10, ATD and ATD GX animals had higher levels of dopamine, DOPAC, and noradrenaline in CING and AID than normal males. It would appear that T acts prenatally through its metabolite, estradiol, to modulate the development of catecholamine activity in the frontal cortex in the neonatal period.
我们之前报道过，新生大鼠暴露于睾酮（T）会减缓其幼崽前皮质中儿茶酚胺能活性的发育进程。在本研究中，我们评估了孕期T在此发育过程中的作用，并探讨了T的雌激素代谢产物雌二醇在这些作用中的角色。在妊娠第10至21天期间，每天皮下注射5 mg/kg芳香化酶抑制剂1,4,6 - 雄甾三烯 - 3,17 - 二酮（ATD）或仅注射赋形剂的母鼠所生的雄性幼崽，在出生后6小时内要么进行性腺切除（GX），要么仅进行麻醉。在出生后（PN）第4天和第10天，使用高效液相色谱 - 电化学检测法（HPLC - EC）评估从扣带回（CING）、无颗粒岛叶（AID）、顶叶（PAR）和枕叶（OC）皮质采集的打孔样本中的多巴胺、3,4 - 二羟基苯乙酸（DOPAC）和去甲肾上腺素水平。在PN4时，处理对胺水平没有影响，尽管额叶区域的水平较高。在PN10时，ATD组和ATD GX组动物的CING和AID中的多巴胺、DOPAC和去甲肾上腺素水平高于正常雄性。似乎T在产前通过其代谢产物雌二醇来调节新生儿期额叶皮质中儿茶酚胺活性的发育。
