佛波酯抑制促甲状腺激素预处理的FRTL-5细胞中白细胞介素-6诱导的DNA合成。

Phorbol ester inhibits DNA synthesis induced by interleukin-6 in TSH-pretreated FRTL-5 cells.

作者信息

Nishiyama S, Takano T, Takada K, Tada H, Amino N

机构信息

Department of Laboratory Medicine, Osaka University School of Medicine, Japan.

出版信息

Endocr Res. 1994 May;20(2):193-200. doi: 10.3109/07435809409030408.

DOI:10.3109/07435809409030408

PMID:8055833

Abstract

Combination of TSH and Interleukin-6 (IL-6) can stimulate DNA synthesis in FRTL-5 cells. Phorbol 12-myristate 13-acetate (PMA) stimulates G0 to G1 transition but inhibits G1 to S transition in these cells. We studied the effect of IL-6 on DNA synthesis in TSH-pretreated FRTL-5 cells in the presence or absence of PMA. IL-6 induced DNA synthesis when it was added to TSH-pretreated cells. Further, PMA inhibited the DNA synthesis induced by IL-6. These data might suggest that in FRTL-5 cells IL-6 can show its mitogenic effect in G1 phase of their cell cycle progression.

摘要

促甲状腺激素（TSH）与白细胞介素-6（IL-6）联合可刺激FRTL-5细胞中的DNA合成。佛波酯12-肉豆蔻酸酯13-乙酸酯（PMA）可刺激这些细胞从G0期转变为G1期，但会抑制其从G1期转变为S期。我们研究了在有或无PMA存在的情况下，IL-6对TSH预处理的FRTL-5细胞中DNA合成的影响。当将IL-6添加到TSH预处理的细胞中时，它可诱导DNA合成。此外，PMA可抑制IL-6诱导的DNA合成。这些数据可能表明，在FRTL-5细胞中，IL-6在其细胞周期进程的G1期可表现出促有丝分裂作用。