Wojtech E, Brimacombe R, Häckel A, Prochnow D, Fasold H
Institut für Biochemie, J. W. Goethe-Universität Frankfurt, Germany.
Eur J Biochem. 1994 Aug 1;223(3):799-803. doi: 10.1111/j.1432-1033.1994.tb19055.x.
Poly(adenylic acid) was modified by methylchlorotetrolic ester in a reproducible and defined content of the derivatized bases. The nucleic acid derivative is protein reactive and was coupled to 70S ribosomes from Escherichia coli, in order to identify proteins along the mRNA pathway. The binding of the label becomes specific under the direction of tRNA(Lys) and is then almost exclusively located on the small subunit. The proteins S1, S12, S18 and S21 were labeled, as shown by an antibody assay. The yield of the affinity label was 5.4%, as calculated from the labeled nucleic acid. This compares favourably with the yields from photolabile compounds.
聚腺苷酸通过甲基氯代四醇酯进行修饰，修饰碱基的含量具有可重复性和确定性。该核酸衍生物具有蛋白质反应活性，并与大肠杆菌的70S核糖体偶联，以鉴定沿mRNA途径的蛋白质。在tRNA（Lys）的指导下，标记物的结合变得具有特异性，然后几乎只位于小亚基上。抗体检测表明，蛋白质S1、S12、S18和S21被标记。根据标记的核酸计算，亲和标记的产率为5.4%。这与光不稳定化合物的产率相比具有优势。
