Fueyo A, Uría J A, Freije J M, López-Otín C
Departamento de Biología Funcional, Facultad de Medicina, Universidad de Oviedo, Spain.
Gene. 1994 Aug 5;145(2):245-9. doi: 10.1016/0378-1119(94)90014-0.
We report here the nucleotide (nt) sequence of a rat liver cDNA encoding Zn-alpha 2-glycoprotein (Zn-alpha 2-gp), a plasma protein with a high degree of sequence similarity to class-I major histocompatibility complex (MHC) antigens. The deduced amino acid (aa) sequence contains the coding information for 293 aa residues and shows 60% identity with the aa sequence of human Zn-alpha 2-gp and 35% identity with that corresponding to the extracellular domains of RT1, a rat class-I MHC antigen. Northern blot analysis showed that rat Zn-alpha 2-gp is expressed in liver, but not in a wide number of tissues, including prostate, mammary gland, kidney, intestine, lung, pancreas, ovary, uterus, thyroid, placenta, spleen, brain and heart.
我们在此报告编码锌α2-糖蛋白（Zn-α2-gp）的大鼠肝脏cDNA的核苷酸（nt）序列，Zn-α2-gp是一种血浆蛋白，与I类主要组织相容性复合体（MHC）抗原具有高度的序列相似性。推导的氨基酸（aa）序列包含293个氨基酸残基的编码信息，与人Zn-α2-gp的氨基酸序列具有60%的同一性，与大鼠I类MHC抗原RT1的细胞外结构域对应的序列具有35%的同一性。Northern印迹分析表明，大鼠Zn-α2-gp在肝脏中表达，但在包括前列腺、乳腺、肾脏、肠道、肺、胰腺、卵巢、子宫、甲状腺、胎盘、脾脏、大脑和心脏在内的多种组织中不表达。
