Stasiv Y Z, Mashkova T D, Chernov B K, Sokolova I V, Itkes A V, Kisselev L L
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
Gene. 1994 Aug 5;145(2):267-72. doi: 10.1016/0378-1119(94)90018-3.
A human cDNA clone encoding a c-myc promoter-binding protein (IRLB) was selected by screening a human fibroblast lambda gt11 phage library with the hexamer oligodeoxyribonucleotide (oligo) 5'-GGCGGGAAAAAGAACGGA, corresponding to the protein-binding element of human c-myc similar to the interferon-stimulated response element (ISRE). The lambda gt11 phage clone, encoding a fusion protein which bound the probe oligo, was used to create an strain of Escherichia coli. The deduced amino-acid sequence of the cloned protein contains a putative alpha-helix which is expected to act as the DNA-binding domain. DNase footprinting analysis and oligo-binding specificity assays showed that the cloned factor recognizes the ISRE-like element of the P2 promoter region of human c-myc.
