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[双酚氧化酶(DPOx)在不同动物物种各组织中的分布(作者译)]

[Distribution of diphenoloxidases (DPOx) in various tissues of various animal species (author's transl)].

作者信息

Tuil D, Berthelon M, Demos J

出版信息

Clin Chim Acta. 1975 Jun 2;61(2):219-27. doi: 10.1016/0009-8981(75)90318-6.

Abstract

An oxidative activity has been demonstrated in different tissues of various animals species (man, rabbit, hen, rat, mouse) measuring it by means of adrenaline, by starch gel electrophoresis and acrylamide gel electrofocusing, followed by incubation of these gel with DOPA. Kidney, liver and erythrocytes of rabbit, rat, mouse and hen, human erythrocytes and platelets display a high oxidative activity. This activity, which is not completely inhibited by KCN, is found in one band on starch gel electrophoresis and in two distinct bands on electrofocusing in the case of platelets and erythrocytes of man, rabbit and rat. The starch gel electrophoretic migration and the electrofocusing pattern are tissue and species dependent.

摘要

通过肾上腺素、淀粉凝胶电泳和丙烯酰胺凝胶电聚焦法,并随后将这些凝胶与多巴一起孵育,已在多种动物物种(人、兔、鸡、大鼠、小鼠)的不同组织中证实了一种氧化活性。兔、大鼠、小鼠和鸡的肾脏、肝脏和红细胞,以及人类红细胞和血小板均表现出高氧化活性。这种活性不完全受氰化钾抑制,在人、兔和大鼠的血小板和红细胞中,淀粉凝胶电泳显示为一条带,电聚焦显示为两条不同的带。淀粉凝胶电泳迁移和电聚焦图谱取决于组织和物种。

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