Alfinito F, Lamberti A, Capobianchi M R, Nigro M, De Rosa G, Tassone P, Turco M C, Dianzani F, Venuta S
Divisione di Ematologia, II School of Medicine, University of Napoli, Italy.
Leukemia. 1994 Aug;8(8):1294-300.
Interferon-gamma (IFN-gamma) has previously been described as exerting a growth factor activity for murine and human stimulated normal T lymphocytes, in addition to its established role in regulating the cytotoxic activity of T and NK cells. We analyzed the effect of human recombinant IFN-gamma on the proliferation of leukemic lymphocytes isolated from the peripheral blood of a patient affected by a T-cell chronic lymphocytic leukemia (T-CLL). Incubation with IFN-gamma induced the proliferation of unstimulated leukemic cells. Cell proliferation was maximal after 6 days of culture with the cytokine; the half-maximal effect of IFN-gamma was observed at a concentration of approximately 800 U/ml. We also measured the production of IFN-gamma by leukemic cells. Cells incubated in control medium released small quantities of IFN-gamma activity, while the addition of low doses of the exogenous cytokine to the cell cultures induced high levels of IFN-gamma mRNA and protein production. Furthermore, anti-HLA class I monoclonal antibodies, that exert a mitogenic effect on these neoplastic lymphocytes, also induced the IFN-gamma gene expression in the same cells. These results indicate that IFN-gamma may stimulate the proliferation of human neoplastic T cells and suggest that this cytokine might have a role in the expansion of T-leukemic cell clones in vivo.
γ干扰素（IFN-γ）此前已被描述为除了在调节T细胞和NK细胞的细胞毒性活性方面具有既定作用外，还对小鼠和人类受刺激的正常T淋巴细胞发挥生长因子活性。我们分析了重组人IFN-γ对从一名T细胞慢性淋巴细胞白血病（T-CLL）患者外周血中分离出的白血病淋巴细胞增殖的影响。用IFN-γ孵育可诱导未受刺激的白血病细胞增殖。用该细胞因子培养6天后细胞增殖达到最大值；在浓度约为800 U/ml时观察到IFN-γ的半数最大效应。我们还检测了白血病细胞产生IFN-γ的情况。在对照培养基中孵育的细胞释放少量的IFN-γ活性，而向细胞培养物中添加低剂量的外源性细胞因子可诱导高水平的IFN-γ mRNA和蛋白产生。此外，对这些肿瘤淋巴细胞发挥促有丝分裂作用的抗HLA-I类单克隆抗体也可诱导相同细胞中的IFN-γ基因表达。这些结果表明，IFN-γ可能刺激人类肿瘤性T细胞的增殖，并提示该细胞因子可能在体内T白血病细胞克隆的扩增中起作用。
