Younes M, Katikaneni P R, Lechago L V, Lechago J
Department of Pathology, Baylor College of Medicine, Methodist Hospital, Houston, Texas.
Mod Pathol. 1994 Apr;7(3):396-400.
HAM56, a monoclonal antibody first used to identify macrophages and endothelial cells, also stains many carcinomas (except those arising in the digestive tract). This property is useful in the differentiation between primary ovarian and metastatic colonic carcinomas in the ovary, or between gynecological (ovarian and endometrial) and colonic implants and lymph node metastases. This distinction is important as the prognoses of colorectal and gynecological malignancies differ significantly. Sixteen primary ovarian carcinomas (10 with peritoneal implants and 3 with lymph node metastases), eight cases of primary colonic carcinomas (four metastatic to ovary, four with peritoneal implants, and four with lymph node metastases), and three primary endometrial carcinomas, all with metastases to the ovary, were immunostained with the HAM56 antibody using the ABC immunoperoxidase technique. Linear membranous immunostaining was considered positive, whereas staining of mucin and debris was regarded as negative. Using these parameters, 15/16 ovarian primaries, 9/10 ovarian implants, 3/3 ovarian lymph node metastases, and 3/3 endometrial primaries and their ovarian metastases were positive. Colonic primaries, their ovarian metastases, peritoneal implants, and lymph node metastases were all negative. It is concluded that the HAM56 antibody is a useful tool in the distinction between colorectal and ovarian malignancies in those cases where the routine histological appearance may be ambiguous.
HAM56是一种单克隆抗体，最初用于识别巨噬细胞和内皮细胞，它也能使许多癌组织（消化道起源的癌除外）染色。这一特性有助于鉴别原发性卵巢癌与卵巢转移性结肠癌，或鉴别妇科（卵巢和子宫内膜）植入物及淋巴结转移灶与结肠植入物及淋巴结转移灶。这种鉴别很重要，因为结直肠癌和妇科恶性肿瘤的预后有显著差异。采用ABC免疫过氧化物酶技术，用HAM56抗体对16例原发性卵巢癌（10例有腹膜植入物，3例有淋巴结转移）、8例原发性结肠癌（4例转移至卵巢，4例有腹膜植入物，4例有淋巴结转移）以及3例均有卵巢转移的原发性子宫内膜癌进行免疫染色。线性膜状免疫染色被视为阳性，而黏液和碎片的染色则视为阴性。根据这些参数，16例原发性卵巢癌中有15例、10例卵巢植入物中有9例、3例卵巢淋巴结转移中有3例以及3例原发性子宫内膜癌及其卵巢转移灶均呈阳性。结肠癌原发性肿瘤、其卵巢转移灶、腹膜植入物及淋巴结转移灶均为阴性。结论是，在常规组织学表现可能不明确的情况下，HAM56抗体是鉴别结直肠癌和卵巢恶性肿瘤的有用工具。
