Nagaki M, Sasaki T, Shimura S, Satoh M, Takishima T, Shirato K
First Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
Respir Physiol. 1994 May;96(2-3):311-9. doi: 10.1016/0034-5687(94)90135-x.
Submucosal glands were isolated from feline trachea. The intracellular Ca2+ concentration ([Ca2+]i) of the acinar cells of isolated glands was measured using the fluorescent dye Fura-2. Calcitonin gene-related peptide (CGRP) at 10(-8) to 10(-5) M produced a significant and sustained rise in the [Ca2+]i of isolated glands, reaching a maximal response of 127% of the prior baseline level but did not alter the intracellular adenosine 3', 5'-cyclic monophosphate ([cAMP]i). In a Ca(2+)-free solution, CGRP produced no significant alteration in [Ca++]i. Glycoconjugate secretion from isolated glands was stimulated by CGRP in a dose dependent fashion, reaching a maximal response of 167% of control at 10(-6) M but was without effect in tracheal explants. Further, CGRP did not produce any significant increase in glycoconjugate secretion in the Ca(2+)-free medium. These findings indicate that CGRP stimulates glycoconjugate secretion from airway submucosal glands by inducing Ca2+ influx from the extracellular solution.
从猫的气管中分离出黏膜下腺。使用荧光染料Fura - 2测量分离出的腺体腺泡细胞内的Ca2 +浓度（[Ca2 +]i）。10(-8)至10(-5) M的降钙素基因相关肽（CGRP）使分离出的腺体的[Ca2 +]i显著且持续升高，达到先前基线水平的127%的最大反应，但并未改变细胞内3', 5'-环磷酸腺苷（[cAMP]i）。在无Ca(2+)溶液中，CGRP对[Ca++]i无显著影响。CGRP以剂量依赖性方式刺激分离出的腺体分泌糖缀合物，在10(-6) M时达到对照的167%的最大反应，但对气管外植体无作用。此外，在无Ca(2+)培养基中，CGRP不会使糖缀合物分泌有任何显著增加。这些发现表明，CGRP通过诱导细胞外溶液中的Ca2 +内流来刺激气道黏膜下腺分泌糖缀合物。
