一种使用聚合酶链反应和三种引物进行定点诱变的高产方法。 - Suppr

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超能文献

A high-yield method for site-directed mutagenesis using polymerase chain reaction and three primers.

作者信息

Steinberg R A, Gorman K B

机构信息

Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City 73190.

出版信息

Anal Biochem. 1994 May 15;219(1):155-7. doi: 10.1006/abio.1994.1246.

DOI:10.1006/abio.1994.1246

PMID:8059944

Abstract

摘要

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引用本文的文献

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相似文献

A high-yield method for site-directed mutagenesis using polymerase chain reaction and three primers.

Anal Biochem. 1994 May 15;219(1):155-7. doi: 10.1006/abio.1994.1246.

Structure-function studies based on in vitro expression.

Methods Mol Biol. 1995;37:317-35. doi: 10.1385/0-89603-288-4:317.

Enzymatic inverse PCR: a restriction site independent, single-fragment method for high-efficiency, site-directed mutagenesis.

Biotechniques. 1992 Aug;13(2):214-20.

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Methods Mol Biol. 1997;67:183-8. doi: 10.1385/0-89603-483-6:183.

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Methods Mol Biol. 2002;192:189-96. doi: 10.1385/1-59259-177-9:189.

Uracil-USE, an improved method for site-directed mutagenesis on double-stranded plasmid DNA.

Biotechniques. 1995 Mar;18(3):370-2.

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Methods Mol Biol. 2002;192:141-51. doi: 10.1385/1-59259-177-9:141.

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Methods Mol Biol. 2002;192:207-16. doi: 10.1385/1-59259-177-9:207.

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Methods Mol Biol. 1996;57:239-48. doi: 10.1385/0-89603-332-5:239.

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PCR Methods Appl. 1994 Dec;4(3):S131-6. doi: 10.1101/gr.4.3.s131.

引用本文的文献

The inflammation-induced down-regulation of plasma Fetuin-A (alpha2HS-Glycoprotein) in liver results from the loss of interaction between long C/EBP isoforms at two neighbouring binding sites.

Nucleic Acids Res. 2003 Oct 15;31(20):5957-70. doi: 10.1093/nar/gkg788.

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J Physiol. 1995 Dec 15;489 ( Pt 3)(Pt 3):709-21. doi: 10.1113/jphysiol.1995.sp021085.

A high-yield method for site-directed mutagenesis using polymerase chain reaction and three primers.

作者信息

Steinberg R A, Gorman K B

机构信息

Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City 73190.

出版信息

Anal Biochem. 1994 May 15;219(1):155-7. doi: 10.1006/abio.1994.1246.

DOI:10.1006/abio.1994.1246

PMID:8059944

Abstract

摘要