Yuan W, Cawley G F, Eyer C S, Backes W L
Department of Pharmacology and Experimental Therapeutics, Louisiana State University Medical Center, New Orleans 70112-1393.
Biochem Biophys Res Commun. 1994 Aug 15;202(3):1259-65. doi: 10.1006/bbrc.1994.2066.
Rats were treated with a single intraperitoneal injection of ethylbenzene in corn oil and the effects on cytochrome P450 3A-dependent activities, immunoreactive protein levels and RNA levels were examined. Ethylbenzene increased both P450 3A-dependent 2 beta-hydroxylation of testosterone and immunoreactive protein levels. These levels were maximally induced by 24 hr and diminished thereafter. Despite the increase in P450 3A protein, neither P450 3A1 nor P450 3A2 mRNA levels were altered by treatment with the hydrocarbon. These results clearly demonstrate that this P450 isozyme can be induced by either translational activation or stabilization of P450 3A protein and are suggestive of an elevation of P450 3A2 levels.
给大鼠腹腔注射一次玉米油中的乙苯，然后检测其对细胞色素P450 3A依赖性活性、免疫反应性蛋白水平和RNA水平的影响。乙苯增加了睾酮的P450 3A依赖性2β-羟化作用以及免疫反应性蛋白水平。这些水平在24小时时诱导达到最大值，此后下降。尽管P450 3A蛋白增加，但用该碳氢化合物处理后，P450 3A1和P450 3A2的mRNA水平均未改变。这些结果清楚地表明，这种P450同工酶可通过P450 3A蛋白的翻译激活或稳定作用来诱导，提示P450 3A2水平升高。
