Rosselli M, Imthurm B, Macas E, Keller P J, Dubey R K
Department of Obstetrics and Gynecology, University Hospital Zurich, Switzerland.
Biochem Biophys Res Commun. 1994 Aug 15;202(3):1543-52. doi: 10.1006/bbrc.1994.2107.
Ovarian dysfunction as well as impaired release of endothelium-derived nitric oxide (NO) is associated with increased incidence of cardiovascular disease in postmenopausal women. Estrogen replacement therapy in postmenopausal women has a cardioprotective effect. Hence, using follicular development (FD) phase of the menstrual cycle of normal women (n = 7) and of patients undergoing in vitro fertilization (n = 16), we tested whether ovarian/sex hormones modulate NO-release. Levels of nitrite/nitrate (stable metabolites of NO), 17 beta-estradiol (E), progesterone (P), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) were measured in serum collected during FD-phase. Serum nitrite/nitrate levels increased (p < .01) with the normal, as well as hormonally-induced FD and correlated with increases in serum E levels (r2 = 0.67; p < .01), but not with P, FSH and LH (p > .05). Post-ovulatory increase in P and LH levels decreased serum nitrite/nitrate levels (p < .05), even in presence of high E levels. In conclusion, nitrite/nitrate increase with follicular development, an effect that is potentially mediated by E induced NO release.
卵巢功能障碍以及内皮源性一氧化氮（NO）释放受损与绝经后女性心血管疾病发病率增加有关。绝经后女性的雌激素替代疗法具有心脏保护作用。因此，我们利用正常女性（n = 7）和接受体外受精的患者（n = 16）月经周期的卵泡发育（FD）期，测试了卵巢/性激素是否调节NO释放。在FD期采集的血清中测量亚硝酸盐/硝酸盐（NO的稳定代谢产物）、17β-雌二醇（E）、孕酮（P）、卵泡刺激素（FSH）和黄体生成素（LH）的水平。正常以及激素诱导的卵泡发育过程中，血清亚硝酸盐/硝酸盐水平升高（p < 0.01），且与血清E水平升高相关（r2 = 0.67；p < 0.01），但与P、FSH和LH无关（p > 0.05）。排卵后P和LH水平升高会降低血清亚硝酸盐/硝酸盐水平（p < 0.05），即使在E水平较高的情况下也是如此。总之，亚硝酸盐/硝酸盐水平随卵泡发育而升高，这一效应可能由E诱导的NO释放介导。
