Endre Z H, Cowin G J, Stewart-Richardson P, Cross M, Willgoss D A, Duggleby R G
Department of Medicine, University of Queensland, Royal Brisbane Hospital, Australia.
Biochem Biophys Res Commun. 1994 Aug 15;202(3):1639-44. doi: 10.1006/bbrc.1994.2121.
Protection against hypoxic injury by supraphysiological glycine and alanine concentrations was investigated in the isolated perfused rat kidney (IPRK). 23Na NMR detects consistent increases in total renal Na in IPRK during hypoxic perfusion. Increasing the concentration of glycine and alanine to 5 mM each produced a 34% (p < 0.001) reduction in the increase in total renal Na following 30 minutes of hypoxia compared to a matched control group supplemented with 5 mM each of serine and glutamine. There was also a trend (p = 0.067) to improvement in the fractional excretion of sodium (FENa) in the glycine plus alanine treated group. Hypoxic alterations of other physiological parameters were not prevented by supraphysiological glycine plus alanine. This suggests that monitoring total renal Na is a more sensitive method of defining renal injury and protection than monitoring changes in FENa, fractional excretion of potassium (FEK) and inulin clearance.
在离体灌注大鼠肾脏（IPRK）中研究了超生理浓度的甘氨酸和丙氨酸对缺氧损伤的保护作用。23Na核磁共振检测到在缺氧灌注期间IPRK中总肾钠持续增加。与补充5 mM丝氨酸和谷氨酰胺的匹配对照组相比，将甘氨酸和丙氨酸的浓度分别提高到5 mM，在缺氧30分钟后，总肾钠的增加减少了34%（p < 0.001）。在甘氨酸加丙氨酸处理组中，钠分数排泄（FENa）也有改善的趋势（p = 0.067）。超生理浓度的甘氨酸加丙氨酸并不能防止其他生理参数的缺氧改变。这表明监测总肾钠是一种比监测FENa、钾分数排泄（FEK）和菊粉清除率变化更敏感的定义肾损伤和保护的方法。
