Burghardt H, Brunner H, Oelmüller R, Lottspeich F, Oster U, Rüdiger W
Botanisches Institut, Universität München, Bundesrepublik Deutschland.
Z Naturforsch C J Biosci. 1994 May-Jun;49(5-6):321-6. doi: 10.1515/znc-1994-5-607.
Cress (Lepidium sativum) seeds were germinated in darkness. Seedlings were investigated for soluble proteins by SDS-PAGE. Two proteins were identified by microsequencing: the small subunit of ribulosebisphosphate carboxylase (SSU) and the alpha subunit of the storage protein cruciferin. Net synthesis of small and large subunits of ribulosebisphosphate carboxylase (SSU and LSU) was investigated by Western blot. Net synthesis of both subunits was inhibited by coumarin. To the contrary, net synthesis of cruciferin was increased by coumarin. With specific cDNA probes, we determined steady state levels of the corresponding mRNAs (rbcS mRNA for SSU, rbcL mRNA for LSU). Both mRNAs can be detected in dry seeds; their amount increases during germination in the dark. Incubation with coumarin inhibits this increase. Inhibition of development by coumarin on the level of transcription is discussed.
