Notterpek L M, Rome L H
Department of Biological Chemistry, UCLA School of Medicine 90024-1737.
Neuron. 1994 Aug;13(2):473-85. doi: 10.1016/0896-6273(94)90361-1.
A direct role for neurons in CNS myelination has yet to be demonstrated. CNS myelination can be examined in cerebellar slice cultures, which faithfully reproduce both synthesis and wrapping of myelin. In an attempt to demonstrate a role for axolemma in this process, we generated more than 2000 axolemma-reactive monoclonal antibodies. One clone, G21.3, repeatedly blocked myelination in cerebellar slices, as documented by both biochemistry and morphology. The antibody caused a dramatic reduction in myelin lipid and protein synthesis. CNS white matter, sciatic nerve, and neuronal cultures were positively stained with G21.3, whereas oligodendrocytes and myelin were fully negative. The antibody identified a restricted number of proteins in purified axolemma. These results suggest a direct involvement of axons in CNS myelination.
中枢神经系统（CNS）中神经元在髓鞘形成过程中的直接作用尚未得到证实。可以在小脑切片培养物中研究中枢神经系统的髓鞘形成，这种培养物能如实地再现髓鞘的合成和包裹过程。为了证明轴膜在此过程中的作用，我们制备了2000多种抗轴膜反应性单克隆抗体。其中一个克隆G21.3反复阻断小脑切片中的髓鞘形成，这在生物化学和形态学上都得到了证实。该抗体导致髓鞘脂质和蛋白质合成显著减少。中枢神经系统白质、坐骨神经和神经元培养物被G21.3阳性染色，而少突胶质细胞和髓鞘则完全呈阴性。该抗体在纯化的轴膜中识别出数量有限的蛋白质。这些结果表明轴突直接参与了中枢神经系统的髓鞘形成。
