脂肪酸诱导的Ca(2+)依赖性解偶联以及NADH氧化外部途径的激活与环孢菌素A敏感的线粒体通透性转换相关联。

Fatty acid-induced Ca(2+)-dependent uncoupling and activation of external pathway of NADH oxidation are coupled to cyclosporin A-sensitive mitochondrial permeability transition.

作者信息

Starkov A A, Markova O V, Mokhova E N, Arrigoni-Martelli E, Bobyleva V A

机构信息

A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russia.

出版信息

Biochem Mol Biol Int. 1994 Apr;32(6):1147-55.

PMID:8061632

Abstract

Permeabilization of inner mitochondrial membrane by palmitic acid in the presence of Ca2+ (cyclosporin A-sensitive stimulation of respiration, decrease of delta psi and high amplitude swelling) is accompanied by activation of the external pathway of NADH oxidation in liver mitochondria. The "pore"-sealing agents (cyclosporin A, Mg2+ with ADP, and L-carnitine with ATP) are equally effective in preventing the induction of external pathway of NADH oxidation by Ca2+ with palmitate. However, activities of these agents are different in respect to recoupling of permeabilized mitochondria. Participation of cyclosporin A-sensitive "pore" in the fatty acid- and Ca(2+)-dependent induction of external pathway of NADH oxidation and in Ca(2+)-dependent uncoupling is discussed.

摘要

在钙离子存在的情况下，棕榈酸可使线粒体内膜通透化（环孢素A敏感的呼吸刺激、膜电位降低和高幅度肿胀），同时伴有肝脏线粒体中NADH氧化外部途径的激活。“孔道”封闭剂（环孢素A、Mg2+与ADP以及L-肉碱与ATP）在防止钙离子与棕榈酸盐诱导NADH氧化外部途径方面同样有效。然而，这些试剂在使通透化线粒体重新偶联方面的活性有所不同。文中讨论了环孢素A敏感的“孔道”在脂肪酸和钙离子依赖性诱导NADH氧化外部途径以及钙离子依赖性解偶联中的作用。