关于ppGpp和pppGpp体外合成的研究。

Studies on the in vitro synthesis of ppGpp and pppGpp.

作者信息

Beres L, Lucas-Lenard J

出版信息

Biochim Biophys Acta. 1975 Jun 2;395(1):80-90. doi: 10.1016/0005-2787(75)90236-1.

Abstract

The effect of a number of inhibitors of protein synthesis on ppGpp and pppGpp synthesis in vitro has been examined. As expected from in vivo results, chloramphenicol is without effect on this reaction. Aurintricarboxylic acid and chlortetracycline, on the other hand rapidly and specifically inhibit ppGpp synthesis. Fusidic acid in the presence of saturating amounts of EF G also inhibits the reaction completely, suggesting that an empty ribosomal acceptor site is necessary for this reaction. On the other hand, the 50-S subunit proteins L7 and L12 are not required for stringent factor activity. Ribosomes from Pseudomonas fluorescens can replace those from Escherichia coli in the complete system, while ribosomes from Ehrlich ascites cannot. A small but reproducible synthesis of ppGpp is observed when the ribosomal wash from E. coli is complemented with ribosomes from wheat germ cytoplasm.

摘要

已经研究了多种蛋白质合成抑制剂对体外ppGpp和pppGpp合成的影响。正如体内实验结果所预期的那样，氯霉素对该反应没有影响。另一方面，金精三羧酸和金霉素能迅速且特异性地抑制ppGpp的合成。在存在饱和量EF G的情况下，夫西地酸也能完全抑制该反应，这表明该反应需要一个空的核糖体受体位点。另一方面，50-S亚基蛋白L7和L12对于严谨因子活性并非必需。在完整系统中，荧光假单胞菌的核糖体可以替代大肠杆菌的核糖体，而艾氏腹水瘤的核糖体则不能。当用小麦胚芽细胞质的核糖体补充大肠杆菌的核糖体洗涤液时，可观察到少量但可重复的ppGpp合成。