一种基于分光光度法的灵敏方法，用于测定生物系统中过氧化氢的生成速率。

A sensitive spectrophotometry-based method for the determination of the rate of hydrogen peroxide generation in biological systems.

作者信息

Di Paolo M L, Scarpa M, Rigo A

机构信息

Department of Biological Chemistry, University of Padova, Italy.

出版信息

J Biochem Biophys Methods. 1994 Apr;28(3):205-14. doi: 10.1016/0165-022x(94)90017-5.

DOI:10.1016/0165-022x(94)90017-5

PMID:8064115

Abstract

A new sensitive spectrophotometric method for the determination of the rate of hydrogen peroxide generation in biological systems has been developed. This method is based on the measurement of the oxidation rate of reduced cytochrome c by H2O2 in the presence of a mediator and permits the detection of H2O2 generation rates as low as 60 nM min-1. The solution of the differential equations of the kinetic process permitted the calculation of the kinetic rate constants and assessment of the conditions required to measure the hydrogen peroxide generation rate.

摘要

已开发出一种用于测定生物系统中过氧化氢生成速率的新型灵敏分光光度法。该方法基于在介质存在下通过H2O2对还原型细胞色素c的氧化速率进行测量，并能够检测低至60 nM min-1的H2O2生成速率。动力学过程微分方程的求解使得动力学速率常数得以计算，并可评估测量过氧化氢生成速率所需的条件。