Scott A I
Department of Chemistry, Texas A&M University, College Station 77843-3255.
J Nat Prod. 1994 May;57(5):557-73. doi: 10.1021/np50107a001.
The feasibility of multi-step, one-flask total synthesis of natural products is demonstrated by cloning and overexpression of the corresponding biosynthetic enzymes from plant and microbial sources. As many as eight or nine enzymes can be recombined in vitro to reach an advanced intermediate in the vitamin B12 pathway.
通过克隆和过量表达来自植物和微生物来源的相应生物合成酶，证明了天然产物多步、单瓶全合成的可行性。在维生素B12途径中，多达八种或九种酶可以在体外重组以得到一个高级中间体。
