Hunter M G, Biggs C, Pickard A R, Faillace L S
Department of Physiology and Environmental Science, Faculty of Agricultural and Food Sciences, University of Nottingham, UK.
J Reprod Fertil. 1994 May;101(1):139-44. doi: 10.1530/jrf.0.1010139.
Two experiments were carried out in which follicular aromatase activity was compared between Meishan and Large-White hybrid gilts. In Expt 1, preovulatory follicles (n = 10 largest per animal) were recovered from Meishan and Large-White hybrid gilts (n = 5 per breed) on the day before predicted onset of behavioural oestrus, and the granulosa cells and theca tissue incubated to determine aromatase activity. Follicles recovered from Meishan pigs were smaller (P < 0.01) and contained fewer granulosa cells (P < 0.05), but follicular oestradiol content of the breeds was similar (P > 0.1). Aromatase activity was higher in the theca tissue (P < 0.05) and tended to be higher in the granulosa cells recovered from Meishan follicles (P = 0.065). In Expt 2, granulosa cell aromatase activity was investigated during the early follicular phase (estimated day 16 of cycle) in Meishan and Large-White hybrid gilts (n = 6 and 5, respectively). The number of follicles > or = 1 mm diameter recovered per animal was 171 for both breeds (P > 0.1), whereas the number of follicles > or = 2 mm diameter was 65 and 101 (P < 0.05) from Meishan and Large-White hybrid gilts, respectively. The mean diameter of all follicles recovered was smaller in the Meishan gilts (P < 0.001). Overall, neither the number of granulosa cells per follicle, as indicated by DNA estimation, nor the oestradiol content differed between the breeds at this time (P > 0.1).(ABSTRACT TRUNCATED AT 250 WORDS)
进行了两项实验，比较了梅山猪与大白杂交母猪的卵泡芳香化酶活性。在实验1中，于预计行为发情开始前一天，从梅山猪和大白杂交母猪（每个品种n = 5）中采集排卵前卵泡（每头动物10个最大卵泡），将颗粒细胞和卵泡膜组织进行培养以测定芳香化酶活性。从梅山猪采集的卵泡较小（P < 0.01），颗粒细胞数量较少（P < 0.05），但两个品种的卵泡雌二醇含量相似（P > 0.1）。卵泡膜组织中的芳香化酶活性较高（P < 0.05），从梅山猪卵泡中回收的颗粒细胞中的芳香化酶活性也有升高趋势（P = 0.065）。在实验2中，研究了梅山猪和大白杂交母猪（分别为n = 6和5）卵泡早期（估计周期第16天）颗粒细胞的芳香化酶活性。每个动物回收的直径≥1 mm的卵泡数量，两个品种均为171个（P > 0.1），而直径≥2 mm的卵泡数量，梅山猪和大白杂交母猪分别为65个和101个（P < 0.05）。梅山猪回收的所有卵泡的平均直径较小（P < 0.001）。总体而言，此时两个品种之间，无论是通过DNA估计的每个卵泡颗粒细胞数量，还是雌二醇含量，均无差异（P > 0.1）。（摘要截短于250字）
