侵袭性曲霉病——一种兔模型
Invasive aspergillosis--a rabbit model.
作者信息
Abdul Samad S, Yasin M S, Arumugham G, Yap K L
机构信息
Department of Medical Microbiology and Immunology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur.
出版信息
Malays J Pathol. 1993 Dec;15(2):119-23.
An invasive aspergillosis model in rabbits was attempted using 3 concentrations of A. fumigatus conidia. Conidia concentrations of 1 x 10(6), 1 x 10(7) and 1 x 10(8) were inoculated intravenously into rabbits. The severity of infection was directly proportional to the inoculum size of the conidia. Aspergillus fumigatus was isolated from livers, kidneys, spleens, hearts and lungs of infected rabbits at a rate of 82%, 75%, 57%, 54% and 32% respectively. Cultures of urine specimens taken by bladder tap were positive for A. fumigatus in 30% of the rabbits tested. Blood cultures using the Bactec Fungal System (Becton Dickinson Corp., USA) failed to isolate A. fumigatus in 20 rabbits with biopsy-proven invasive apergillosis. Active infection with high fungal tissue burden occurred between 2-4 days after infection in rabbits inoculated with 1 x 10(7) conidia.
尝试使用3种浓度的烟曲霉分生孢子建立兔侵袭性曲霉病模型。将浓度为1×10⁶、1×10⁷和1×10⁸的分生孢子静脉注射到兔体内。感染的严重程度与分生孢子的接种量直接成正比。分别从感染兔的肝脏、肾脏、脾脏、心脏和肺中分离出烟曲霉，分离率分别为82%、75%、57%、54%和32%。通过膀胱穿刺采集的尿液标本培养物中，30%受试兔的烟曲霉呈阳性。使用Bactec真菌系统（美国贝克顿·迪金森公司）对20只经活检证实为侵袭性曲霉病的兔进行血培养，未能分离出烟曲霉。接种1×10⁷分生孢子的兔在感染后2至4天出现伴有高真菌组织负荷的活动性感染。
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