Nyquist-Battie C, Hagler K E, Love S
School of Biological Sciences, University of Missouri, Kansas City 64108.
Life Sci. 1994;55(9):653-9. doi: 10.1016/0024-3205(94)00671-7.
The aim of the present work was to determine the effect of abdominal aortic stenosis on molecular forms of acetylcholinesterase (AChE) in rat heart. Pressure-overload, left ventricular hypertrophy was produced in male Sprague-Dawley rats by suprarenal abdominal aortic constriction. After two weeks the relative heart weight was increased over 20% compared to sham-surgical controls, mostly due to left ventricular enlargement. Aortic constriction reduced AChE activity per wet weight and per unit protein by 25-30% in the left ventricle and interventricular septum, but not in the other chambers. However, total AChE activity per chamber was normal in the left ventricle and interventricular septum, but was elevated in the atria. The molecular forms of AChE were separated in linear sucrose gradients and their specific activities were calculated from the resulting percent activities and total AChE activities. This data showed that although aortic constriction had no effect on ratios of the various forms, it did reduce the specific activities of globular and asymmetric forms in the left ventricle and interventricular septum. The reduced AChE activity suggests that slower rates of ACh hydrolysis occur in the left ventricle in pressure-overload hypertrophy.
本研究的目的是确定腹主动脉狭窄对大鼠心脏中乙酰胆碱酯酶（AChE）分子形式的影响。通过肾上腺上方腹主动脉缩窄在雄性Sprague-Dawley大鼠中产生压力超负荷性左心室肥厚。两周后，与假手术对照组相比，相对心脏重量增加超过20%，这主要是由于左心室扩大。主动脉缩窄使左心室和室间隔中每湿重和每单位蛋白的AChE活性降低25%-30%，但在其他心腔中未降低。然而，左心室和室间隔中每个心腔的总AChE活性正常，但心房中的总AChE活性升高。AChE的分子形式在线性蔗糖梯度中分离，并根据所得的活性百分比和总AChE活性计算其比活性。该数据表明，尽管主动脉缩窄对各种形式的比例没有影响，但它确实降低了左心室和室间隔中球形和不对称形式的比活性。AChE活性降低表明在压力超负荷性肥厚的左心室中，ACh水解速率减慢。
