Xia Y, Fertel R H, Wood J D
Department of Physiology, College of Medicine, Ohio State University Columbus 43210.
Life Sci. 1994;55(9):685-92. doi: 10.1016/0024-3205(94)00675-x.
Effects of 5-hydroxytryptamine (5-HT) and related agonists and antagonists on formation of cAMP were determined for enzymatically dissociated ganglia from the myenteric plexus of the guinea-pig small intestine. Formation of cAMP was stimulated by 5-HT in both dose- and time- dependent manners. The stimulatory action of 5-HT was suppressed by the 5-HT1P antagonist, renzapride, but not by the 5-HT3 antagonist, tropisetron (formerly ICS 205-930). Neither renzapride nor cisapride increased the levels of cAMP. Levels of cAMP were suppressed by concentrations of tropisetron greater than 1.0 microM. Levels of cAMP were unaffected by the 5-HT3 agonist, 2-methyl-5-hydroxytryptamine. A putative 5-HT4 receptor agonist, 5-methoxytryptamine, stimulated formation of cAMP, but to a lesser extent than 5-HT. We conclude that 5-HT acts to stimulate adenylate cyclase and the formation of cAMP in myenteric ganglia. The 5-HT1P receptor is the most likely subtype involved in 5-HT action on cAMP formation.
针对豚鼠小肠肌间神经丛经酶解离的神经节，测定了5-羟色胺（5-HT）及相关激动剂和拮抗剂对环磷酸腺苷（cAMP）形成的影响。5-HT以剂量和时间依赖性方式刺激cAMP的形成。5-HT1P拮抗剂伦扎必利可抑制5-HT的刺激作用，但5-HT3拮抗剂托烷司琼（原ICS 205-930）则无此作用。伦扎必利和西沙必利均未增加cAMP水平。浓度大于1.0微摩尔的托烷司琼可抑制cAMP水平。5-HT3激动剂2-甲基-5-羟色胺对cAMP水平无影响。一种假定的5-HT4受体激动剂5-甲氧基色胺可刺激cAMP的形成，但程度低于5-HT。我们得出结论，5-HT可刺激肌间神经节中的腺苷酸环化酶和cAMP的形成。5-HT1P受体最有可能是参与5-HT对cAMP形成作用的亚型。
