霍乱弧菌自发释放的菌体抗原的部分纯化及特性
Partial purification and properties of somatic antigen spontaneously released from Vibrio cholerae.
作者信息
Pike R M, Chandler C H
出版信息
Infect Immun. 1975 Jul;12(1):187-92. doi: 10.1128/iai.12.1.187-192.1975.
The supernatant fluids from cultures of Vibrio cholerae grown for 40 h in an dialyzable medium were dialyzed, concentrated, and fractionated on agarose columns. The fractions containing most of the antigen which inhibited the vibriocidal activity of homologous antiserum were pooled, dialyzed, and concentrated to provide material with about 100 times the specific activity of the original culture supernatant. This material, containing 12 to 20% protein, 11 to 19% carbohydrate, and about 16% unbound lipid, had a mean lethal dose for mice of about 500 mug. This partially purified antigen absorbed all the vibriocidal antibody from homologous antiserum against live cultures and produced a single line of precipitation in gel diffusion tests with the same antiserum.
将霍乱弧菌在可透析培养基中培养40小时后所得的培养上清液进行透析、浓缩，并在琼脂糖柱上进行分级分离。收集含有大部分抑制同源抗血清杀弧菌活性抗原的级分，进行透析和浓缩，以提供比原始培养上清液比活性高约100倍的物质。该物质含12%至20%的蛋白质、11%至19%的碳水化合物和约16%的游离脂质，对小鼠的平均致死剂量约为500微克。这种部分纯化的抗原从针对活培养物的同源抗血清中吸收了所有杀弧菌抗体，并在与同一种抗血清的凝胶扩散试验中产生了一条沉淀线。
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