Kennedy J R, Richardson S H
J Bacteriol. 1969 Dec;100(3):1393-401. doi: 10.1128/jb.100.3.1393-1401.1969.
The fine structural changes associated with cell growth and toxin production have been examined in Vibrio cholerae strain 569B. No morphological alterations in the cell envelope are apparent during logarithmic growth with thin-section techniques. However, internal swelling, suggesting alteration of cell envelope permeability, is evident particularly during the late logarithmic and early stationary phases of growth. Certain extracellular material demonstrable with negative-stain techniques does appear during the period of toxin production. The possible origin of this material is discussed. The effects of high temperature (37 C) and aeration on cell structure are also examined.
已对霍乱弧菌569B菌株中与细胞生长和毒素产生相关的精细结构变化进行了研究。采用超薄切片技术观察到，在对数生长期，细胞包膜没有明显的形态学改变。然而，特别是在对数生长期后期和生长稳定期早期，细胞内部出现肿胀，提示细胞包膜通透性发生改变。在毒素产生期间，确实出现了某些能用负染技术显示的细胞外物质。本文讨论了这种物质可能的来源。还研究了高温（37℃）和通气对细胞结构的影响。
