Kinoshita I, Huard J, Tremblay J P
Laboratoire de Neurobiologie, Université Laval, Hôpital de l'Enfant-Jésus, Québec, Canada.
Muscle Nerve. 1994 Sep;17(9):975-80. doi: 10.1002/mus.880170903.
A possible treatment for Duchenne muscular dystrophy is the injection of normal myoblasts into dystrophic muscles to induce the formation of new, healthy, and dystrophin-positive muscle fibers. To develop this therapy, it is important to identify the muscle fibers formed by the injected myoblasts in the host muscles. In this study, we used myoblasts from transgenic mice which have a gene expressing beta-galactosidase under the control of the promoter of quail fast skeletal muscle troponin I. This transgene is expressed in myotubes and muscle fibers, but not in myoblasts. Twenty-eight days after myoblast transplantation in nude and in mdx mice, muscle fibers containing of beta-galactosidase were identified by x-gal staining. In mdx mice, most of the beta-galactosidase-positive muscle fibers resulting from the myoblast transplantation were also dystrophin positive. This technique could make it possible to follow the success of myoblast transplantation even in mice that are not depleted of dystrophin.
杜氏肌营养不良症的一种可能治疗方法是将正常的成肌细胞注射到营养不良的肌肉中，以诱导形成新的、健康的、抗肌萎缩蛋白阳性的肌纤维。为了开发这种疗法，识别宿主肌肉中由注射的成肌细胞形成的肌纤维很重要。在本研究中，我们使用了来自转基因小鼠的成肌细胞，这些小鼠具有一个在鹌鹑快骨骼肌肌钙蛋白I启动子控制下表达β-半乳糖苷酶的基因。该转基因在肌管和肌纤维中表达，但不在成肌细胞中表达。在裸鼠和mdx小鼠中进行成肌细胞移植28天后，通过x-gal染色鉴定出含有β-半乳糖苷酶的肌纤维。在mdx小鼠中，成肌细胞移植产生的大多数β-半乳糖苷酶阳性肌纤维也是抗肌萎缩蛋白阳性的。即使在没有抗肌萎缩蛋白缺失的小鼠中，这种技术也可以追踪成肌细胞移植的成功情况。
