Hao R, Cerutis D R, Blaxall H S, Rodriguez-Sierra J F, Pfeiffer R F, Ebadi M
Section of Neurology, University of Nebraska College of Medicine, Omaha 68198-6260.
Neurochem Res. 1994 Jun;19(6):761-7. doi: 10.1007/BF00967717.
Metallothionein (MT) isoforms I and II were first identified and characterized in our laboratories in several regions of brain, in hippocampal neurons in primary culture, and in retinoblastoma and neuroblastoma cell lines. In this study, by having employed the MT-I cDNA as a probe, we sought to gain additional insight about the function of MT by discerning the regional distribution of its mRNA. Northern blot analyses of brain mRNA revealed that the administration of zinc enhanced dramatically MT-I mRNA (570 bp). The in situ hybridization study revealed that MT-I mRNA was located in several areas of brain, with the highest concentrations found in the cerebellum, hippocampus, and ventricles. The results of these studies are interpreted to suggest that zinc enhances the synthesis of MT mRNA and MT in turn may participate in zinc associated functions in neurons.
金属硫蛋白（MT）同工型I和II最初是在我们实验室中，于脑的几个区域、原代培养的海马神经元以及视网膜母细胞瘤和神经母细胞瘤细胞系中被鉴定和表征的。在本研究中，我们以MT-I cDNA作为探针，试图通过识别其mRNA的区域分布来进一步了解MT的功能。对脑mRNA的Northern印迹分析表明，锌的给药显著增强了MT-I mRNA（570 bp）。原位杂交研究表明，MT-I mRNA位于脑的几个区域，在小脑、海马和脑室中浓度最高。这些研究结果被解释为表明锌增强了MT mRNA的合成，而MT反过来可能参与神经元中与锌相关的功能。
