Andersen R D, Birren B W, Ganz T, Piletz J E, Herschman H R
DNA. 1983;2(1):15-22. doi: 10.1089/dna.1.1983.2.15.
Starting with liver mRNA from cadmium chloride-treated rats, we constructed a cDNA library. A mouse metallothionein 1 (MT-1) cDNA restriction fragment (Durnam et al. 1980) was used as a hybridization probe to isolate from this library a cDNA (p2A10) which is a nearly complete copy of the rat MT-1 mRNA sequence. Southern analysis of rat DNA, using p2A10 as a probe, together with the screening of a rat genomic library, reveal that the rat MT-1 gene belongs to a family of related sequences. The rat MT-1 gene is inducible both by glucocorticoids and by metals.
我们从用氯化镉处理过的大鼠肝脏mRNA开始构建了一个cDNA文库。用小鼠金属硫蛋白1（MT-1）cDNA限制性片段（Durnam等人，1980年）作为杂交探针，从该文库中分离出一个cDNA（p2A10），它几乎是大鼠MT-1 mRNA序列的完整拷贝。用p2A10作为探针进行大鼠DNA的Southern分析，以及对大鼠基因组文库的筛选表明，大鼠MT-1基因属于一个相关序列家族。大鼠MT-1基因可被糖皮质激素和金属诱导。
