Wadzinski A M, Ribbons D W
J Bacteriol. 1975 Jul;123(1):380-1. doi: 10.1128/jb.123.1.380-381.1975.
Methanomonas methanooxidans incorporates both carbon atoms of acetate into the glutamate and aspartate families of amino acids during growth on methane; carbon dioxide is also evolved from both carbon atoms of acetate. The distribution of carboxyl-labeled acetate incorporated into convalently bound glutamate is consistent with the operation of the tricarboxylic acid cycle in this species, and the presence of alpha-ketoglutarate dehydrogenase was demonstrated in cell-free extracts.
在以甲烷为碳源生长过程中,氧化甲烷单胞菌将乙酸盐的两个碳原子都并入谷氨酸和天冬氨酸氨基酸家族;乙酸盐的两个碳原子也都会释放出二氧化碳。掺入共价结合谷氨酸中的羧基标记乙酸盐的分布与该物种中三羧酸循环的运作一致,并且在无细胞提取物中证实了α-酮戊二酸脱氢酶的存在。