Hiley C G, Otter M, Bell J, Strange R C, Keeling J W
Clinical Biochemistry Research Laboratory, School of Postgraduate Medicine, Keele University, North Staffordshire Hospital, Stoke on Trent, England.
Pediatr Pathol. 1994 May-Jun;14(3):497-504. doi: 10.3109/15513819409024279.
We describe immunohistochemical studies of the expression of alpha and pi class glutathione S-transferases (GSTs) in normal fetal kidneys. These define, in greater detail, changes in expression of alpha isoforms in the proximal tubule. At about 36 weeks of gestation expression of alpha isoforms was down-regulated in the distal tubules and collecting ducts while pi was expressed throughout the nephron. Tubular expression of alpha isoforms was restricted to the part adjacent to the glomerulus; cells farthest from the glomerulus were negative. After 40 weeks of gestation, alpha isoforms were expressed along the entire proximal tubule, while pi was restricted to the distal tubule and collecting ducts. GST expression was also studied in multicystic renal dysplasia, autosomal recessive polycystic kidney disease, and autosomal dominant polycystic kidney disease to determine whether the patterns of expression of alpha and pi isoforms allow identification of the origin of the cysts that characterize these diseases. Cysts were lined by epithelia that were strongly positive for alpha and pi isoforms. The epithelia of noncystic nephrons in renal cystic dysplasia demonstrated delayed maturity, suggesting that GST expression was dependent on the stage of development and not length of gestation.
我们描述了正常胎儿肾脏中α和π类谷胱甘肽S-转移酶（GSTs）表达的免疫组织化学研究。这些研究更详细地界定了近端小管中α同工型表达的变化。在妊娠约36周时，α同工型在远端小管和集合管中的表达下调，而π在整个肾单位中均有表达。α同工型的小管表达局限于与肾小球相邻的部分；离肾小球最远的细胞呈阴性。妊娠40周后，α同工型沿整个近端小管表达，而π局限于远端小管和集合管。还对多囊性肾发育不良、常染色体隐性多囊肾病和常染色体显性多囊肾病中的GST表达进行了研究，以确定α和π同工型的表达模式是否有助于识别这些疾病特征性囊肿的起源。囊肿内衬上皮对α和π同工型呈强阳性。肾囊性发育不良中非囊肿性肾单位的上皮显示成熟延迟，提示GST表达取决于发育阶段而非妊娠时长。
