Edelstein L M, Cariglia N, Okun M R, Patel R P, Smucker D
J Invest Dermatol. 1975 May;64(5):364-70. doi: 10.1111/1523-1747.ep12512294.
Melanosomal "tyrosinase" (L-dopa) was isolated from trypsin digest of B-16 mouse melanoma melanosomes, using polyacrylamide gel disc electrophoresis. The enzyme was represented by a single band, having characteristics similar to the T1 dopa-positive band observed when using supernatants of crude melanoma homogenates as the source. When gels with this band were incubated in solutions containing tyrosine and dopa in varying ratios , there was no enhancement of melanin formation by tyrosine when compared with incubations in corresponding concentrations of dopa alone. These data further support previous studies in our laboratory demonstrating an inability of so-called mamalian "tyrosinase" to convert tyrosine to melanin; since this enzyme readily converts L-dopa to melanin, it seems more reasonable to term this enzyme an L-dopa oxidase.
使用聚丙烯酰胺凝胶圆盘电泳从B - 16小鼠黑色素瘤黑素小体的胰蛋白酶消化物中分离出黑素体“酪氨酸酶”（L - 多巴）。该酶表现为单一谱带，其特性与以粗制黑色素瘤匀浆上清液为来源时观察到的T1多巴阳性谱带相似。当含有该谱带的凝胶在含有不同比例酪氨酸和多巴的溶液中孵育时，与仅在相应浓度的多巴中孵育相比，酪氨酸并未增强黑色素的形成。这些数据进一步支持了我们实验室之前的研究，该研究表明所谓的哺乳动物“酪氨酸酶”无法将酪氨酸转化为黑色素；由于这种酶能轻易地将L - 多巴转化为黑色素，将这种酶称为L - 多巴氧化酶似乎更合理。
