Sung P
Sealy Center for Molecular Science, University of Texas Medical Branch at Galveston 77555-1061.
Science. 1994 Aug 26;265(5176):1241-3. doi: 10.1126/science.8066464.
The RAD51 gene of Saccharomyces cerevisiae is required for genetic recombination and DNA double-strand break repair. Here it is demonstrated that RAD51 protein pairs circular viral single-stranded DNA from phi X 174 or M13 with its respective homologous linear double-stranded form. The product of synapsis between these DNA partners is further processed by RAD51 to yield nicked circular duplex DNA, which indicates that RAD51 can catalyze strand exchange. The pairing and strand exchange reaction requires adenosine triphosphate, a result consistent with the presence of a DNA-dependent adenosine triphosphatase activity in RAD51 protein. Thus, RAD51 is a eukaryotic recombination protein that can catalyze the strand exchange reaction.
酿酒酵母的RAD51基因对于基因重组和DNA双链断裂修复是必需的。本文证明，RAD51蛋白能将来自φX 174或M13的环状病毒单链DNA与其各自同源的线性双链形式进行配对。这些DNA配对物之间的联会产物会被RAD51进一步加工，产生带切口的环状双链DNA，这表明RAD51能够催化链交换。配对和链交换反应需要三磷酸腺苷，这一结果与RAD51蛋白中存在依赖于DNA的三磷酸腺苷酶活性一致。因此，RAD51是一种能够催化链交换反应的真核重组蛋白。
