由感染SP82的枯草芽孢杆菌的RNA聚合酶在体内和体外产生的转录本的DNA链特异性。
DNA strand specificity of transcripts produced in vivo and in vitro by RNA polymerase from SP82-infected Bacillus subtilis.
作者信息
Lawrie J M
出版信息
J Virol. 1975 May;15(5):1286-8. doi: 10.1128/JVI.15.5.1286-1288.1975.
Abstract
Phage-specific RNA synthesized early in the infection of Bacillus subtilis with SP82 hybridizes to both heavy (H) and light (L) strands of SP82 DNA nearly equally. Phage RNA synthesized during the middle of the infection hybridizes preferentially to the H strand. The ratio of H/L strand binding of RNAs synthesized in vitro by RNA polymerases isolated from uninfected and infected cells resembles the ratios of early and middle phage RNA classes, respectively. This supports the conclusion that a modified RNA polymerase is required for the transcription of middle RNA classes.
摘要
用SP82感染枯草芽孢杆菌早期合成的噬菌体特异性RNA几乎同等程度地与SP82 DNA的重链（H）和轻链（L）杂交。感染中期合成的噬菌体RNA优先与H链杂交。从未感染和感染细胞中分离出的RNA聚合酶在体外合成的RNA的H/L链结合比率分别类似于早期和中期噬菌体RNA类别的比率。这支持了这样的结论，即中期RNA类别的转录需要一种经过修饰的RNA聚合酶。
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