Solymossy M, Csuka I, Antoni F, Temesi A
First Institute of Biochemistry, Semmelweis University Medical School, Budapest, Hungary.
Acta Physiol Hung. 1993;81(4):327-39.
The uptake of Leu-OMe and Leu-Leu-OMe was studied in vitro in porcine PMN cells. Both methylesters are metabolized leading to the intracellular accumulation of leucine. Part of the hydrolyzed leucine gradually filtrates back into the culture medium in a time-, temperature- and methylester substrate concentration-dependent manner. Another portion of Leu-OMe is converted to Leu-Leu dipeptide. With respect to the cellular effects of Leu-OMe treatment ultrastructural studies showed the presence of large vacuoles without significant alteration of cell viability. Increased exocytosis of lysosomal enzymes did not lead to lytic events. Changes in the plasma membrane are indicated by the observation that Leu-OMe treatment causes the loss of the chemotactic activity to formyl-Met-Leu-Phe.
在猪中性粒细胞中对亮氨酸甲酯（Leu-OMe）和亮氨酸-亮氨酸甲酯（Leu-Leu-OMe）的摄取进行了体外研究。两种甲酯均被代谢，导致亮氨酸在细胞内积累。部分水解的亮氨酸以时间、温度和甲酯底物浓度依赖性方式逐渐回渗到培养基中。另一部分Leu-OMe转化为亮氨酸-亮氨酸二肽。关于Leu-OMe处理的细胞效应，超微结构研究显示存在大液泡，而细胞活力无明显改变。溶酶体酶胞吐作用增加并未导致细胞溶解事件。观察到Leu-OMe处理导致对甲酰甲硫氨酰-亮氨酰-苯丙氨酸的趋化活性丧失，这表明质膜发生了变化。
