用碳盘超微电极上的方波阳极溶出伏安法测定血液中的铅。

Determination of lead in blood by square wave anodic stripping voltammetry at a carbon disk ultramicroelectrode.

作者信息

Feldman B J, Osterloh J D, Hata B H, D'Alessandro A

机构信息

University of California, San Francisco General Hospital 94110.

出版信息

Anal Chem. 1994 Jul 1;66(13):1983-7. doi: 10.1021/ac00085a010.

DOI:10.1021/ac00085a010

PMID:8067521

Abstract

A method for blood lead ([Pb]) analysis is developed based on square wave anodic stripping voltammetry (SWASV) in an Hg(2+)-containing solution using a 10-microns-diameter carbon disk ultramicroelectrode. SWASV eliminates interference from O2 in unsparged blood-derived sample solutions, and filtration of acidified samples through nitrocellulose reduces the concentration of interfering substances, increasing the size and improving the uniformity of peak currents. Blood [Pb] values obtained by SWASV are compared with blood [Pb] values determined by graphite furnace atomic absorption spectrophotometry; good accuracy and reasonable coefficients of variation in the range 10.6-20% (average of 13.5%) are found for blood [Pb] ranging from 4 to 30 micrograms/dL. Preliminary experiments using a carbon disk microarray electrode suggest future improvements in sensitivity.

摘要

基于方波阳极溶出伏安法（SWASV），在含Hg(2+)的溶液中使用直径为10微米的碳盘超微电极开发了一种血液铅（[Pb]）分析方法。SWASV消除了未除气的血液衍生样品溶液中O2的干扰，通过硝酸纤维素对酸化样品进行过滤降低了干扰物质的浓度，增加了峰电流的大小并提高了其均匀性。将通过SWASV获得的血液[Pb]值与通过石墨炉原子吸收分光光度法测定的血液[Pb]值进行比较；发现对于4至30微克/分升的血液[Pb]，具有良好的准确性，变异系数在10.6-20%范围内（平均为13.5%）。使用碳盘微阵列电极的初步实验表明未来灵敏度会有所提高。