Volm M, Sauerbrey A, Zintl F
German Cancer Research Center, Heidelberg.
Anticancer Res. 1994 May-Jun;14(3B):1377-81.
The expression of thymidylate-synthase (TS) and dihydrofolate-reductase (DHFR) was analysed immunohistochemically in 110 initial and 28 relapsed childhood acute lymphoblastic leukemia (ALL). Relapse-rates were not significantly higher in initial ALL with overexpression of TS- and DHFR than in ALL without overexpression of the proteins. In addition, the disease-free survival was not different for patients with TS or DHFR-negative and TS- or DHFR-positive ALL. No differences were found in the overall survival times of patients with relapsed ALL and with or without expression of TS or DHFR. Of the initial ALL 42% showed TS-overexpression, whereas of the relapsed ALL 64% of the cases were TS-positive (p = 0.03). The corresponding values for DHFR were 20% and 32% (p = 0.18). The results demonstrate clearly that expression of the proteins TS and DHFR has no importance for the resistance of ALL although the proteins increase during treatment.
采用免疫组织化学方法分析了110例初发和28例复发的儿童急性淋巴细胞白血病（ALL）中胸苷酸合成酶（TS）和二氢叶酸还原酶（DHFR）的表达情况。TS和DHFR过表达的初发ALL的复发率并不显著高于未过表达这些蛋白的ALL。此外，TS或DHFR阴性及TS或DHFR阳性ALL患者的无病生存率并无差异。复发ALL患者中，TS或DHFR表达与否的总生存时间也无差异。初发ALL中42%表现为TS过表达，而复发ALL中64%的病例TS呈阳性（p = 0.03）。DHFR的相应数值分别为20%和32%（p = 0.18）。结果清楚地表明，尽管TS和DHFR蛋白在治疗期间增加，但它们的表达对ALL的耐药性并无影响。
