一种适用于在大肠杆菌中进行定点诱变和多亚基蛋白高水平表达的噬菌体载体。 - Suppr

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超能文献

A bacteriophage vector suitable for site-directed mutagenesis and high-level expression of multisubunit proteins in E. coli.

作者信息

Tan S, Conaway R C, Conaway J W

机构信息

Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City.

出版信息

Biotechniques. 1994 May;16(5):824-6, 828.

PMID:8068336

Abstract

摘要

相似文献

A bacteriophage vector suitable for site-directed mutagenesis and high-level expression of multisubunit proteins in E. coli.一种适用于在大肠杆菌中进行定点诱变和多亚基蛋白高水平表达的噬菌体载体。

Biotechniques. 1994 May;16(5):824-6, 828.

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An efficient plasmid vector for constitutive high-level expression of foreign genes in Escherichia coli.一种用于在大肠杆菌中组成型高效表达外源基因的质粒载体。

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High-throughput screening for soluble recombinant expressed kinases in Escherichia coli and insect cells.在大肠杆菌和昆虫细胞中对可溶性重组表达激酶进行高通量筛选。

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Cloning of the glpK gene of Escherichia coli K-12 and its overexpression using a 'sleeper' bacteriophage vector.大肠杆菌K-12的glpK基因克隆及其使用“潜伏”噬菌体载体的过表达。

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[Construction of pMH, a convenient Escherichia coli protein expression vector].[便捷的大肠杆菌蛋白表达载体pMH的构建]

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Efficient site-directed mutagenesis using an overlap extension-PCR method for expressing Mycoplasma hyopneumoniae genes in Escherichia coli.利用重叠延伸聚合酶链反应方法进行高效定点诱变以在大肠杆菌中表达猪肺炎支原体基因

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TFIIH action in transcription initiation and promoter escape requires distinct regions of downstream promoter DNA.转录起始和启动子逃逸过程中TFIIH的作用需要下游启动子DNA的不同区域。

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The inducible elongin A elongation activation domain: structure, function and interaction with the elongin BC complex.可诱导的延伸蛋白A延伸激活结构域：结构、功能及其与延伸蛋白BC复合物的相互作用。

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Identification of a decay in transcription potential that results in elongation factor dependence of RNA polymerase II.鉴定转录潜能的衰减，该衰减导致RNA聚合酶II对延伸因子的依赖性。

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