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本文引用的文献

1
The increment of SII-facilitated transcript cleavage varies dramatically between elongation competent and incompetent RNA polymerase II ternary complexes.SII促进的转录本切割的增量在具有延伸能力和不具有延伸能力的RNA聚合酶II三元复合物之间差异巨大。
J Biol Chem. 1993 Jun 15;268(17):12874-85.
2
SII-facilitated transcript cleavage in RNA polymerase II complexes stalled early after initiation occurs in primarily dinucleotide increments.起始后早期停滞的RNA聚合酶II复合物中，SII促进的转录物切割主要以二核苷酸增量发生。
J Biol Chem. 1993 Jun 15;268(17):12864-73.
3
Elongation factor SII-dependent transcription by RNA polymerase II through a sequence-specific DNA-binding protein.RNA聚合酶II通过一种序列特异性DNA结合蛋白进行的延伸因子SII依赖性转录。
Proc Natl Acad Sci U S A. 1993 Mar 1;90(5):1917-21. doi: 10.1073/pnas.90.5.1917.
4
Transcript cleavage factors from E. coli.来自大肠杆菌的转录物切割因子。
Cell. 1993 Feb 12;72(3):459-66. doi: 10.1016/0092-8674(93)90121-6.
5
Identification of a 3'-->5' exonuclease activity associated with human RNA polymerase II.与人类RNA聚合酶II相关的3'→5'核酸外切酶活性的鉴定。
Proc Natl Acad Sci U S A. 1993 Feb 1;90(3):843-7. doi: 10.1073/pnas.90.3.843.
6
Cloning and identification of testis-specific transcription elongation factor S-II.睾丸特异性转录延伸因子S-II的克隆与鉴定
J Biol Chem. 1994 Jan 28;269(4):3100-3.
7
Purified yeast RNA polymerase II reads through intrinsic blocks to elongation in response to the yeast TFIIS analogue, P37.纯化的酵母RNA聚合酶II在酵母TFIIS类似物P37的作用下，能够通读内在的延伸阻滞。
J Biol Chem. 1994 Jan 14;269(2):936-43.
8
RNA polymerase II transcription factor SIII. I. Identification, purification, and properties.RNA聚合酶II转录因子SIII。I. 鉴定、纯化及特性
J Biol Chem. 1993 Dec 5;268(34):25587-93.
9
Multiple RNA polymerase conformations and GreA: control of the fidelity of transcription.多种RNA聚合酶构象与GreA：转录保真度的控制
Science. 1993 Nov 5;262(5135):867-73. doi: 10.1126/science.8235608.
10
A DNA minor groove-binding ligand both potentiates and arrests transcription by RNA polymerase II. Elongation factor SII enables readthrough at arrest sites.一种DNA小沟结合配体既能增强RNA聚合酶II的转录，又能使其停滞。延伸因子SII能使转录在停滞位点通读。
J Mol Biol. 1994 Feb 25;236(3):725-37. doi: 10.1006/jmbi.1994.1185.

鉴定转录潜能的衰减，该衰减导致RNA聚合酶II对延伸因子的依赖性。

Identification of a decay in transcription potential that results in elongation factor dependence of RNA polymerase II.

作者信息

Gu W, Reines D

机构信息

Graduate Program in Biochemistry & Molecular Biology, Emory University School of Medicine, Atlanta, Georgia 30322, USA.

出版信息

J Biol Chem. 1995 May 12;270(19):11238-44. doi: 10.1074/jbc.270.19.11238.

DOI:10.1074/jbc.270.19.11238
PMID:7744757
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3374591/
Abstract

The rate of RNA elongation by RNA polymerase II (pol II) is affected by DNA sequences called intrinsic arrest sites. Efficient transcription through these sites requires elongation factor SII. In addition to the sequence-specific features of the DNA, we show that the acquisition of SII-dependence is a function of its "dwell-time" at an arrest site. This temperature-dependent decay in elongation potential appears irreversible, implying that factor-dependent and factor-independent elongation complexes are not mutually interconvertible at this position. TFIIF and NH4Cl are known to increase the elongation rate of pol II. Both agents preempt arrest, consistent with the idea that elongation dwell time influences the process. TFIIF and SII act upon different steps in a complementary way to prevent or resolve arrest, respectively. They are probably instrumental in facilitating the efficient transcription of large eukaryotic genes in vivo.

摘要

RNA聚合酶II（pol II）的RNA延伸速率受称为内在终止位点的DNA序列影响。通过这些位点的有效转录需要延伸因子SII。除了DNA的序列特异性特征外，我们还表明，对SII的依赖性的获得是其在终止位点的“停留时间”的函数。这种延伸潜力的温度依赖性衰减似乎是不可逆的，这意味着在该位置，因子依赖性和因子非依赖性延伸复合物不能相互转换。已知TFIIF和NH4Cl可提高pol II的延伸速率。这两种试剂都能防止终止，这与延伸停留时间影响该过程的观点一致。TFIIF和SII分别以互补的方式作用于不同步骤，以防止或解决终止。它们可能有助于在体内促进大型真核基因的有效转录。