Verhaar H J, Damen C A, Duursma S A, Scheven B A
University Hospital of Utrecht, Department of Geriatrics, The Netherlands.
Bone. 1994 May-Jun;15(3):307-11. doi: 10.1016/8756-3282(94)90293-3.
Estrogen/gestagen replacement therapy prevents excess bone loss in postmenopausal women. The mode of action by which these sex steroids exert their anabolic effects on bone has not been completely clarified yet. In this study, 17 beta-estradiol (E2), as well as progestins progesterone (P), dydrogesterone (DD), 20 alpha-dihydroxydydrogesterone (DHD), medroxyprogesterone acetate (MPA), and cyproterone acetate (CPA) were able to stimulate the mitogenesis and differentiation of normal adult human osteoblast-like (HOB) cells harvested from female trabecular bone explants. The different progestins exerted a more pronounced stimulatory effect on HOB proliferation than E2 did. The combination of E2 with P, DD, or DHD did not result in a statistically significant further increase of HOB proliferation, as compared with the progestins alone. In general, E2 showed a stronger differentiation-inducing effect than the progestins, as measured by histochemical staining of the HOB cells for alkaline phosphatase activity. Combining E2 and the progestins did not result in a further increase of the number of alkaline phosphatase positive cells, compared with E2 alone. The different progestins proved to be equally potent in stimulating HOB proliferation and differentiation. In conclusion, progestins as well as E2 exerted anabolic but differential effects on normal adult human osteoblasts in vitro.
雌激素/孕激素替代疗法可预防绝经后女性的骨质过度流失。这些性类固醇对骨骼发挥合成代谢作用的作用方式尚未完全阐明。在本研究中，17β-雌二醇（E2）以及孕激素黄体酮（P）、地屈孕酮（DD）、20α-二羟地屈孕酮（DHD）、醋酸甲羟孕酮（MPA）和醋酸环丙孕酮（CPA）能够刺激从女性小梁骨外植体中获取的正常成人成骨样（HOB）细胞的有丝分裂和分化。与E2相比，不同的孕激素对HOB增殖的刺激作用更为显著。与单独使用孕激素相比，E2与P、DD或DHD联合使用并未导致HOB增殖在统计学上有显著进一步增加。总体而言，通过对HOB细胞进行碱性磷酸酶活性的组织化学染色测定，E2显示出比孕激素更强的诱导分化作用。与单独使用E2相比，E2与孕激素联合使用并未导致碱性磷酸酶阳性细胞数量进一步增加。不同的孕激素在刺激HOB增殖和分化方面被证明具有同等效力。总之，孕激素以及E2在体外对正常成人成骨细胞发挥了合成代谢但不同的作用。
