Hamir A N, Moser G
Laboratory of Large Animal Pathology, University of Pennsylvania, New Bolton Center, Kennett Square 19348.
J Vet Diagn Invest. 1994 Apr;6(2):148-52. doi: 10.1177/104063879400600203.
Although a fast and reliable fluorescent antibody (FA) test for rabies diagnosis is available, ideally the diagnostic procedure requires fresh or frozen brain tissues. In some instances (and particularly for retrospective studies), fresh or frozen tissues may not be available. In such cases, immunohistochemical tests may be utilized. Because such tests have been used only in a limited number of cases, their diagnostic reliability has not been thoroughly evaluated. This study documents the results of a streptavidin-biotin complex (ABC) immunoperoxidase test on formalin-fixed, paraffin-embedded brain tissues of domestic and wild animals that were positive on FA test for rabies prior to fixation. Thirty-nine of 40 rabies cases were positive with the ABC technique. Based on these preliminary results, the ABC technique appears reliable. However, more corroborative test results are needed before the ABC method can be used as a routine diagnostic procedure, especially with field samples and negative controls.
虽然有用于狂犬病诊断的快速可靠的荧光抗体（FA）检测方法，但理想情况下，诊断程序需要新鲜或冷冻的脑组织。在某些情况下（特别是回顾性研究），可能无法获得新鲜或冷冻组织。在这种情况下，可以使用免疫组织化学检测。由于此类检测仅在有限数量的病例中使用过，其诊断可靠性尚未得到充分评估。本研究记录了对固定前FA检测呈狂犬病阳性的家畜和野生动物福尔马林固定、石蜡包埋脑组织进行链霉亲和素-生物素复合物（ABC）免疫过氧化物酶检测的结果。40例狂犬病病例中有39例通过ABC技术检测呈阳性。基于这些初步结果，ABC技术似乎可靠。然而，在ABC方法可作为常规诊断程序使用之前，尤其是对于现场样本和阴性对照，还需要更多的确证性检测结果。
