人类β基因座控制区的高敏位点5发挥着染色质绝缘子的作用。
Hypersensitive site 5 of the human beta locus control region functions as a chromatin insulator.
作者信息
Li Q, Stamatoyannopoulos G
机构信息
Division of Medical Genetics, University of Washington, Seattle 98195.
出版信息
Blood. 1994 Sep 1;84(5):1399-401.
The human beta locus control region (LCR) consists of five DNAse I hypersensitive sites (HS), four of which are erythroid specific and one, the further upstream located 5'HS5, is constitutive. To characterize the function of 5'HS5 we analyzed globin gene expression of various constructs containing HS3 as an enhancer, HS5, and the beta gene as a reporter. Expression was analyzed in stably transfected MEL cells. We found that the enhancing effect of hypersensitive site 3 is blocked when the HS5 is interposed between HS3 and the beta globin gene. These data suggest that the human 5'HS5 has the properties of a chromatin insulator.
人类β基因座控制区（LCR）由五个脱氧核糖核酸酶I超敏位点（HS）组成，其中四个是红系特异性的，另一个位于更上游的5'HS5是组成型的。为了表征5'HS5的功能，我们分析了各种构建体的珠蛋白基因表达，这些构建体包含作为增强子的HS3、HS5以及作为报告基因的β基因。在稳定转染的MEL细胞中分析表达情况。我们发现，当HS5插入HS3和β珠蛋白基因之间时，超敏位点3的增强作用被阻断。这些数据表明，人类5'HS5具有染色质绝缘子的特性。
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