Hartman J C, Olszanski D A, Hullinger T G, Brunden M N
Upjohn Laboratories, Kalamazoo, Michigan.
J Pharmacol Toxicol Methods. 1994 Jun;31(3):153-60. doi: 10.1016/1056-8719(94)90078-7.
The objective of this investigation was to validate a transit-time ultrasound blood flow metering system in vivo. Implanted chronically and acutely on the ascending aorta of the dog, the transit-time flow probe determined varying flow rates simultaneously with measurements made by the electromagnetic flow metering method. The transit-time technique was also compared to two methods in which blood was collected volumetrically by either graduated cylinder (ascending aorta/dog) or pump withdrawal (abdominal aorta/cat). Statistical analysis of the results provided evidence that the transit-time ultrasound method measured in vivo blood flow rate no differently than the electromagnetic or pump withdrawal techniques, however, transit-time determinations of blood volume were 10% below that indicated by graduated cylinder collection. With transit time represented on the y-axis, three linear regressions of all paired blood flow measurements were calculated yielding the following slopes (delta y/delta x) and regression coefficients (r), respectively: electromagnetic (1.00, 0.98), graduated cylinder (0.85, 0.93), and pump withdrawal (0.93, 1.00). The results validate the transit-time ultrasound system used in the present investigation as an accurate method capable of measuring blood flow in both acutely and chronically instrumented animal preparations.
本研究的目的是在体内验证一种渡越时间超声血流测量系统。将渡越时间血流探头长期和短期植入犬的升主动脉，该探头与电磁血流测量法同时测定不同的流速。渡越时间技术还与另外两种方法进行了比较，这两种方法分别是通过量筒（犬升主动脉）或泵抽取（猫腹主动脉）进行体积法采血。对结果的统计分析表明，渡越时间超声法测量的体内血流速率与电磁法或泵抽取技术测量的结果没有差异，然而，渡越时间法测定的血容量比量筒采集法测定的结果低10%。以渡越时间为纵坐标，计算了所有配对血流测量值的三个线性回归，分别得出以下斜率（Δy/Δx）和回归系数（r）：电磁法（1.00，0.98）、量筒法（0.85，0.93）和泵抽取法（0.93，1.00）。结果验证了本研究中使用的渡越时间超声系统是一种能够在急性和慢性仪器化动物制剂中准确测量血流的方法。
