Bosman F T
Department of Pathology, Erasmus University Rotterdam, The Netherlands.
Microsc Res Tech. 1994 Jun 15;28(3):216-25. doi: 10.1002/jemt.1070280306.
In this paper, the use of immunohistochemistry for the analysis of basement membrane components and related extracellular matrix proteins in human cancer is reviewed. Basement membranes in cancer are dynamic structures that are constantly degraded but also deposited, in close collaboration between tumor cells and stromal cells. Basement membrane immunohistochemistry, using antibodies against type IV collagen and laminin, appears to be a useful tool in the analysis of lesions on the borderline between premalignant and malignant. Basement membrane interruptions, however, cannot be used as the only criterion for the diagnosis of malignancy. Type VII collagen is often degraded prior to type IV collagen and laminin in early invasion. This protein also tends to be expressed in carcinomas when it is not found in the corresponding normal tissue. Tenascin seems to play a complex role in the development of human tumors, including promotion of cell growth and differentiation, cell migration during invasion, and tissue remodeling during the development of primary and metastatic lesions. Further systemic exploration of extracellular matrix molecules in neoplasms should yield new information relevant for cancer biologists and useful in cancer diagnosis.
本文综述了免疫组织化学在分析人类癌症中基底膜成分及相关细胞外基质蛋白方面的应用。癌症中的基底膜是动态结构，在肿瘤细胞与基质细胞的密切协作下不断降解但也会沉积。使用抗IV型胶原和层粘连蛋白抗体的基底膜免疫组织化学似乎是分析癌前病变和恶性病变边界处病变的有用工具。然而，基底膜中断不能用作诊断恶性肿瘤的唯一标准。在早期侵袭中，VII型胶原通常比IV型胶原和层粘连蛋白更早降解。这种蛋白在相应正常组织中未发现时，也往往在癌组织中表达。腱糖蛋白似乎在人类肿瘤发展中发挥复杂作用，包括促进细胞生长和分化、侵袭过程中的细胞迁移以及原发性和转移性病变发展过程中的组织重塑。对肿瘤中细胞外基质分子的进一步系统探索应能为癌症生物学家提供新的相关信息，并有助于癌症诊断。
