液相色谱法同时测定绿豆中的有机酸和维生素C

Simultaneous determination of organic acids and vitamin C in green beans by liquid chromatography.

作者信息

Vazquez Oderiz M L, Vazquez Blanco M E, Lopez Hernandez J, Simal Lozano J, Romero Rodriguez M A

机构信息

Universidad de Santiago de Compostela, Facultad de Farmacia, Departmento de Química Analítica, Nutrición y Bromatología, La Coruña, Spain.

出版信息

J AOAC Int. 1994 Jul-Aug;77(4):1056-9.

PMID:8069112

Abstract

A method is described for determining and quantitating organic acids (oxalic, malic, citric, and fumaric) and vitamin C by liquid chromatography with a UV-visible detector that allows simultaneous monitoring at 2 wavelengths. The method was applied to samples of green beans (Phaseolus vulgaris L.). Recoveries were 97.8% for oxalic acid, 98.9% for malic acid, 98.7% for citric acid, 99.2% for fumaric acid, and 98.5% for vitamin C. Method precisions (coefficients of variation) were 1.7% for oxalic acid, 0.8% for malic acid, 0.9% for citric acid, 1.5% for fumaric acid, and 1.2% for vitamin C. Measurement precisions (coefficients of variation) were 1.32% for oxalic acid, 0.33% for malic acid, 0.62% for citric acid, 1.01% for fumaric acid, and 0.39% for vitamin C. Limits of detection were 0.025 mg/mL for oxalic acid, 0.022 mg/mL for malic acid, 0.024 mg/mL for citric acid, 1.0 x 10(-4) mg/mL for fumaric acid, and 2.7 x 10(-4) mg/mL for vitamin C.

摘要

描述了一种通过带有紫外可见检测器的液相色谱法测定和定量有机酸（草酸、苹果酸、柠檬酸和富马酸）及维生素C的方法，该检测器允许在两个波长下同时监测。该方法应用于绿豆（菜豆）样品。草酸的回收率为97.8%，苹果酸为98.9%，柠檬酸为98.7%，富马酸为99.2%，维生素C为98.5%。方法精密度（变异系数）：草酸为1.7%，苹果酸为0.8%，柠檬酸为0.9%，富马酸为1.5%，维生素C为1.2%。测量精密度（变异系数）：草酸为1.32%，苹果酸为0.33%，柠檬酸为0.62%，富马酸为1.01%，维生素C为0.39%。检测限：草酸为0.025mg/mL，苹果酸为0.022mg/mL，柠檬酸为0.024mg/mL，富马酸为1.0×10⁻⁴mg/mL，维生素C为2.7×10⁻⁴mg/mL。